The AcycloPrime-FP method is a simple, homogeneous, robust and high-throughput approach to SNP Detection using microplates and fluorescence polarization detection.

There are four simple steps in this SNP assay:

• Amplify 100-300 bp fragment Genomic DNA containing SNP region of interest
• Degrade Primers/nucleotides with PCR clean up Reagent (containing SNP Primers, R110/Tamra - Acyclo Terminators, unlabeled terminators, AcycloPol), then add AcycloPrime Mix to incorporate dye-Acyclo Terminators
• Perform 10-30 Thermocycles
• Analyze by Fluorescence Polarization, e.g., with the VICTOR² Multilabel Reader

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Comparison of genotyping by two methods. The Results obtained by the AcycloPrime SNP kit (data in green), are superior to that obtained from using Thermosequenase and dideoxynucleotides (data in purple color). Note superior cluster separations by AcycloPrime, making it easier to score genotypes, with greater accuracy.

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AcycloPrime-FP kits are optimized for use with the Victor² and Envision™readers, state-of-the-art multi-use fluorescence polarization detection instruments. By combining the PerkinElmer's SNP Detection systems with the advanced capabilities of these readers, detecting all known SNPs is easy, quick and accurate:

• Proven technology - well published technique adopted by many researchers
• Easy to use - all reagents are provided in the kit along with specific instructions.
• Simple protocol - homogeneous assay with no plate-to-plate transfers, centrifugation, wash or separation steps.
• Better sensitivity and accuracy than other methods - novel AcycloTerminators and AcycloPol Enzyme provide results that are superior to dye-ddNTP's and Thermo Sequenase
• Fast time to results - high throughput read times (fifteen minutes with the Victor² and one minute with the Envision per 384 well plate)
• More affordable than most other technologies - perfect for labs just starting SNP Detection.

 

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Kit Components

All AcycloPrime-FP SNP kits contain:

• PCR Clean-up Reagent, 10X
• PCR Clean-up Dilution Buffer
• 10 X Reaction Buffer
• AcycloTerminator Mix (one pair R110/Tamra dye AcycloTerminators, unlabeled terminators, AcycloPol thermostable polymerase enzyme, and SNP primers which are to be provided by researchers)
• Instruction Manual

Ordering Information

The AcycloPrime™-FP SNP Detection Systems can detect all possible combinations for SNPs. Each kit contains a terminator mix with the appropriate dye-labeled AcycloTerminators that will be incorporated into the SNP primer. These correspond to the alleles present at the SNP to be detected.

Cat. No.	Size	Terminator Mix (R110/TAMRA)
ACP101A	1K SNP Kits (1000 SNPs)	G/A
ACP101B	10K SNP Kit (10,000 SNPs)	G/A
ACP101C	100K SNP Kit (100,000 SNPs)	G/A
ACP102A	1K SNP Kits (1000 SNPs)	G/C
ACP102B	10K SNP Kit (10,000 SNPs)	G/C
ACP102C	100K SNP Kit (100,000 SNPs)	G/C
ACP103A	1K SNP Kits (1000 SNPs)	G/T
ACP103B	10K SNP Kit (10,000 SNPs)	G/T
ACP103C	100K SNP Kit (100,000 SNPs)	G/T
ACP104A	1K SNP Kits (1000 SNPs)	C/A
ACP104B	10K SNP Kit (10,000 SNPs)	C/A
ACP104C	100K SNP Kit (100,000 SNPs)	C/A
ACP106A	1K SNP Kits (1000 SNPs)	C/T
ACP106B	10K SNP Kit (10,000 SNPs)	C/T
ACP106C	100K SNP Kit (100,000 SNPs)	C/T
ACP109A	1K SNP Kits (1000 SNPs)	A/T
ACP109B	10K SNP Kit (10,000 SNPs)	A/T
ACP109C	100K SNP Kit (100,000 SNPs)	A/T
ACP113B	10K SNP Kit (10,000 SNPs)	Includes: 3K G/A, 3K C/T, 1K G/C, 1K G/T, 1K C/A, 1K A/T
ACP113C	100K SNP Kit (100,000 SNPs)	Includes: 30K G/A, 30K C/T, 10K G/C, 10K G/T, 10K C/A, 10K A/T

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1 Chen, X., Levine, L. and Kwok, P., "Fluorescence polarization in homogeneous nucleic acid analysis" Genome Research, (1999) 9, 492-298.

2 Hsu, T.M., Chen, X., Duan, S., Miller, R.D., and Kwok, P-Y., “Universal SNP genotyping assay fluorescence polarization detection”, BioTechniques (2001) 31, 560-570.

3 Gardner, A.F. and Jack, W.E. “Acyclic and dideoxy terminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases”, Nucleic Acids Res. 30, 605-613.

 

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