advances in GPCR
products and
technologies at
PerkinElmer.
Cell as Reagents: Emerging Trends in Utilizing Cell Lines as Reagents – Novel Methods for High Throughput Screening Applications
A review of recent literature devoted to GPCR high throughput screening (HTS) shows an increasing reliance on the use of cell based assays - despite the inherent challenges in their use.
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Scintillation Proximity Assays (SPA) in GPCR Research
Despite numerous advances in nonradioactive technologies, radioligand binding assays remain popular for analysis of ligand-receptor interaction in High Throughput Screening (HTS) and Structure Activity Relationship (SAR) studies in GPCR research.
A well-established radioligand binding assay is Scintillation Proximity Assay (SPA), which due to its homogenous nature (no separation of bound and unbound radiotracers) is easily miniaturized and automated for HTS applications. SPA’s homogeneity allows binding reactions to be assayed without the cumbersome washing or filtration procedures normally used to separate bound from free fractions.
Assays are performed using radioactive labels that emit short range (10 μm) electrons in water. When bound close to a solid scintillator surface by the binding reaction, they produce photons detectable with a scintillation counter. Electrons emitted from labeled molecules not bound close to the surface dissipate their energy in the medium and are not detected. The bound fraction is detected without separation of the solution from the solid support.
New GPCR Application Guide:
The right assays. The right instruments.
Right where you want them. From the initial overview section which helps you visualize our comprehensive offering to the Cell Line Cross-reference Guide this new tool is packed full of information. Starting with our GPCR cell lines, we detail the validation we conduct on all our cell lines, highlight our photoproteins for calcium measurement and end with ready-to-use frozen cells.