Automating HPLC Method Development, Robustness Testing & Validation Duration: 1 hour, 10 minutes
Basic principles and approaches regarding HPLC method development are presented including preliminary considerations, running initial scouting runs, procedures for setting up a series of experiments (a survey), and valuable pointers on evaluating the results. Throughout, the key optimization parameters are discussed and evaluated.
Based on the initial results, suggestions are provided for follow-up experiments, including interpolation routines. These are intended to help gain additional information and possible improvements to the method.
Finally, strategies for method robustness testing are discussed as a part of initial method validation.
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