Tyramide Signal Amplification
A brief history of the TSA technology with a discussion about the chemistry of TSA and how it works. Images with and without TSA of IHC/ISH applications and choice of kits. Troubleshooting tips for high background and No/Low Signal. TSA Web and Technical Support information.
Applicable for UltraView in the LVI product line and the microarray scanners in FGP.
Liquid Scintillation Counting
A basic theory of using the liquid scintillation counting process.
Basic Nuclear Counting:
• Applications
• Benefits
Basic Liquid Scintillation Counting:
• The scintillation process
• Sample Vial + PMT Tubes
• Beta decay
• Beta and antineutrino contribution
Quench, Quench Correction & DPM determination:
• What is Quench?
• Types of quench
• Quench correct methods for DPM measurement
• External standards-Quench curve
LSC Cocktails & Vials:
• Selecting a cocktail
• Glass or plastic vials?
• Conversion to small vials for LSC