The best TR-FRET technology just got better! Introducing new LANCE Ultra. Our proven, cost effective technology provides a toolbox of both kinase-specific and generic reagents to meet all your drug discovery needs—with optimum data results you can trust.

A specific anti-phospho-peptide antibody (Ab) labeled with an europium chelate (Eu) is used in conjunction with a peptide substrate directly labeled with the new ULight acceptor dye for the detection of kinase activity. The binding of the Eu Ab to the phosphorylated ULight peptide substrate brings both the donor and acceptor dye molecules into close proximity. Upon irradiation at 320/340 nm, the excited Eu transfers its energy to the nearby ULight dye molecule, which in turn emits light at 665 nm. The intensity of light emission is proportional to the level of phosphorylation.

• ULight directly-labeled peptides—simplified assay format, superior performance
• Optimized kinase substrate and phospho-detection reagents—easier,
  faster assay development
• Light-resistant ULight dye—ideal for HTS and uHTS applications
• Robust and highly precise— typical Z' values of 0.8 or better
• Entirely scalable—for 96-, 384- and 1536-well microplates
• Optimized for HTS and uHTS—throughputs of up to 1M compounds/day with ViewLux™ Multilabel Plate Imager

We’re so sure you’ll want to try LANCE Ultra we’re offering a free sample to qualified researchers for a limited time!

LANCE Ultra Reagents

PerkinElmer’s Extensive Line of LANCE Reagents and Applications

LANCE Ultra Technology Overview

New LANCE Ultra Reagents for the Development and Optimization of Kinase Assays poster

Comparison of LANCE Ultra with other TR-FRET platforms for a src Tyrosine Kinase Assay poster

LANCE Ultra PKA Kinase Assay technical note

LANCE Ultra MSK1 Kinase Assay technical note

LANCE Ultra p60c-src Kinase Assay - Using ULight-Poly GT technical note