Operetta

Operetta

Offering a new bench-top approach to High Content Screening (HCS), the Operetta™ is easy to use, allowing researchers who are new to HCS to use preconfigured applications and quickly become productive.

 

Designed to provide maximum functionality in the smallest possible footprint, the Operetta occupies minimal space and delivers maximum results. It includes multiple wavelength excitation, brightfield and a wide choice of objectives for maximum flexibility.

 

Complementary to the Opera as an assay development solution it can be easily used as a starter instrument for HCS applications. Preconfigured applications assist researchers who are new to HCS and user configurable building blocks enable more experienced users to develop their own assays.

 

Utilizing an intuitive user interface, the software Harmony™ is designed to streamline and simplify the HCS process. Harmony includes all the tools to develop an assay, automate the hardware, analyze data, and store and retrieve the results. Remote access is available on an office computer via local network to access the Operetta remotely, and then to analyze and view results.

 

Key Features

  • Easy to use solution for high content screening – intuitive user interface, short learning curve and on-line user guides enable fast results.

  • Compact bench-top design fits into any size laboratory.

  • Remote experimental setup and analysis provides the ultimate in system flexibility.

  • Multiple wavelength excitation and brightfield illumination enable versatility.

  • Exceptional image quality with alignment-free illumination.

  • Fast image processing using multi-processor technology.

  • User-selectable objectives provide long working distances and high numerical apertures to match diverse user applications.

  • Powerful image segmentation includes automatic parameter optimization for ease-of-use.

  • Database enabled to provide simple parsing by user, application, date, screen, and compound.


Options

  • The basic Operetta is a four color fluorescence excitation / emission, upgradeable to eight colour excitation

  • Up to 15 emission filters, user changeable

  • Large choice of objectives, 10x to 100x – high NA for maximum performance and long working distance for using any plate type

 

High

Columbus Image Data Management

Automatic parameter adjustment helps you quickly find the best settings. Find images, metadata and results easily in the Harmony database.

 

Product Support

Related Products

Operetta Application Guide – unique Application support

To help new HCS users, we have designed an easy to follow, unique guide to analysing your results. This step-by-step handbook will help you through the process from using a ready-made solution to designing a new image analysis sequence from the building blocks provided.

The guide includes general considerations, recommendations and common pitfalls, so that you can proceed with confidence and produce results quickly.

Harmony Ready-Made Solution

Examples:

  • Cell counting or Nuclei counting
  • Live / dead cell counting
  • Quantification of marker in nucleus, cytoplasm or membrane
  • Cytosol to nucleus translocation
  • Cytosol to membrane translocation
  • Fluorescence redistribution – cytoskeleton
  • Spot analysis
  • Nuclear spots
  • Nuclear analysis - nuclear shrinking
  • Nuclear fragmentation
  • Nuclear classification
  • Cell shape – cell rounding
  • Mitotic index
  • Cell Cycle classification

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