Alliance HIV-1 P24 ANTIGEN ELISA Kit (480 Test)

The PerkinElmer kit provides reagents for immune complex disruption (ICD) of antigen/antibody complexes in serum and plasma samples using a combination of low pH and heat. The samples are then neutralized and transferred to microplate wells which are coated with a highly specific mouse monoclonal antibody to HIV-1 p24. The immobilized monoclonal antibody captures both free HIV-1 p24 and that which has been released upon disruption of immune complexes in the serum/plasma sample. Cell culture samples do not require disruption and are added directly to the monoclonal antibody-coated microplate wells. The captured antigen is complexed with biotinylated polyclonal antibody to HIV-1 p24, followed by a streptavidin-HRP (horseradish peroxidase) conjugate. The resulting complex is detected by incubation with ortho- phenylenediamine-HCl (OPD) which produces a yellow color that is directly proportional to the amount of HIV-1 p24 captured. The absorbance of each microplate well is determined using a microplate reader and calibrated against the absorbance of an HIV-1 p24 antigen standard or standard curve. Samples with absorbance values equal to or greater than the cutoff factor are considered initially reactive, but should be retested in duplicate to determine whether the reactivity is reproducible. Repeatably reactive samples should be tested with PerkinElmer HIV-1 p24 Confirmatory Reagents. The HIV-1 p24 Confirmatory Reagents use a specific antibody neutralization step prior to testing with the HIV-1 p24 ELISA kit to indicate the presence of HIV-1 antigen(s). Samples that are neutralized by the HIV-1 p24 Confirmatory Reagents are considered positive for HIV-1 p24 antigen(s).

This kit is intended for research purposes only and is not approved for diagnostic use.

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