LANCE® TR-FRET Assays

LANCE® TR-FRET Assays

Ideal for HTS and ultra HTS, LANCE biochemical TR-FRET assays are sensitive, homogeneous and easy to use.This antibody-dependant assay provides specificity for proteins within complex signal transduction pathways, can be miniaturized and is readily automatable.

Two formats available:

LANCE Ultra - Designed for kinases LANCE Ultra is a two component system which utilizes a small, red-shifted ULight- dye labeled peptide substrate and a Europium (Eu)-labeled anti-phospho antibody. Direct coupling of the acceptor dye to the target peptides provides faster results and easier development through validated assay pairs.

LANCE - Developed as a generic three component system, this assay uses a Eu-based chelate as the long lifetime donor dye and allophycocyanin-streptavidin (SA-APC) as the acceptor dye. The third component consists of a biotinylated peptide which is recognized by the Eu-labeled antibody and can bind to SA-APC to form the final TR-FRET complex.

LANCE is the biochemcial assay platform you can rely on.