Iodination with Lactoperoxidase, [¹²⁵I]- or [¹³¹I]-

With this method, lactoperoxidase catalyzes the oxidation of iodide using hydrogen peroxide as the enzyme substrate. It is a milder oxidative technique than Chloramine-T, which requires the presence of a tyrosine.

Reference: Markalonis, J.J., Biochem.J. 113:299 (1969).