Iodination with Chloramine-T and Sodium Iodide, [^125I]- or [¹³¹I]-

PerkinElmer offers the Chloramine-T (p-toluene sulfonochloramide) method - the most widely used iodination procedure, and an effective way to label a variety of proteins and peptides such as albumins, globulins, neuropeptides, and chemokines. It is an oxidative method which involves exposure of the substrate to Chloramine-T in the presence of NaI, [¹²⁵I]- or [¹³¹I]- for a short time, producing high specific activity proteins or peptides labeled with carrier-free radioiodine. It is used in applications with a history of success in this labeling method, or where our experience indicates a probability of success.

Reference: Hunter, W.M., and Greenwood, F.C., Nature 194:495 (1962).