cyclic AMP

Upon agonist activation, G protein-coupled receptors (GPCRs) activate membrane enzyme effectors such as adenylyl cyclase (AC) and phospholipase C (PLC) which in turn stimulates the formulation of cAMP and IP3 second messenger molecules.

PerkinElmer offers different products and technologies allowing measurement of levels of cyclic AMP (cAMP) resulting from G_αi and G_αs coupled receptors found on whole cells or receptor membrane preparations. They include:

• cAMP assay kits based on LANCE®, AlphaScreen® and FlashPlate^® technologies
• cAMPZen Frozen Cells

PerkinElmer offers many assay technologies to detect the functionality of GPCRs by measurement of cAMP levels in whole cells and membrane preparations.

• A highly sensitive luminescence homogeneous assay technology that allows high throughput, sensitive functional cAMP assays with a wide dynamic range
• A single technology that can be used to perform cell and membrane based functional cAMP assays

• The industry standard in cAMP measurements utilized by leading HTS labs around the globe. A homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) immunoassay designed to measure cAMP produced upon modulation of adenylyl cyclase activity by GPCRs
• Intended for the detection of cAMP produced by live cells or from cellular membrane preparations stimulated with GPCR agonists
• A simple, flexible, robust and very sensitive cAMP assay that can be used in laboratories where results may be obtained on a variety of multilabel detectors

• LANCE – Low compound inference makes it ideal for uHTS application
• The Adenylyl Cyclase Activation FlashPlate Assay is a fully homogeneous assay kit, optimized to allow measurement of receptor-mediated Adenylyl Cyclase activation by direct measurement of cAMP production from whole cells or membranes.
• For cAMP functional assays HTS.
• A simple protocol that measures a true second messenger response without extraction in an easy-to-automate assay which provides fast and reliable qualification of lead compounds-perfect for HTS applications.