Cytotoxicity/Cell Proliferation Assays

ATP is a marker for cell viability because it is present in all metabolically active cells. Because ATP concentration declines rapidly when cells undergo necrosis or apoptosis, monitoring ATP is a good indicator of cytocidal, cytostatic and proliferation effects.Our ATPlite and ATPlite 1step luminescence assay systems use patented innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin as shown in the scheme below.



Advantages:

• Rapid
  • results generated in 15 - 25 minutes
• Simple and reproducible
  • no separation steps
• Homogeneous assay
  • no cell harvesting or centrifugation required
• Sensitive
  • down to 5 cells in 100 μL medium (derived from CHO and HL-60 cells in 100 μL medium)
• Wide linear dynamic range
  • ≥ 10⁵ ( as derived from CHO and HL-60 cells)